Red mite is one of the most arthropods in layers that has a worldwide distribution. Although there are different synthetic compounds against this mite, because of drug resistance and chemical residuals of these compounds in meat and egg, substitute methods like herbal medicine now are developing. In the following study N-Hexane and Ethanol extract, Artemisia absinthium, and Artemisia annua are used. GC-MS analysis is performed to determine the compounds of these two extracts. Then lethal property on red mite determined by contact toxici-ty. In the field study, ethanolic extract of Artemisia absinthium and Artemisia annua are sprayed on laying hens infected with red mite. This study reveals that the most compound of ethanol and N-hexan extract of Ar-temisia annua was Dimethoxyphenylacetylene and Benzene respectively. thymol and cyclohexane were the major constituents of ethanol and N-hexan extract of Artemisia absinthium respectively. LC50 of ethanol ex-tract of Artemisia absinthium and Artemisia annua was 16 µg/cm3. Field studies show that the ethanolic extract of these two plants is effective on red mite and reduce the population of the red mite. The study shows Ethanol extract of Artemisia absinthium and Artemisia annua could be used as a substitute compound against the red mite.