Chromium (VI) contaminated water has been considered a major environmental concern. In this study, indigenous bacteria were investigated for their effects on the reduction of hexavalent chromium in water. From this sediment, Cr (VI) reduction rates were dependent on temperature. Cr (VI) reduction rates mounted up by reducing primary Cr (VI) concentration. The results showed that pH considerably influenced the rate of Cr (VI) reduction, with faster decrease taking place at pH 7.0. Higher concentrations of CH3COONa decreased the Cr (VI) reduction. These strategies can be considered as efficient for Cr (VI) removal from water because of their very low cost.