Archive \ Volume.7 2018 Issue 3

Phytochemical, Free Radical Scavenging and Antimicrobial Activities of Ceratonia Siliqua L. Fruits Collected of Jijel

A. Benbott , C. Mosbah , Y. Moumen
Abstract

The purpose of this study is to realize a phytochemical screening and determine the antioxidant and antibacterial activities of the phenolic compound of Ceratonia siliqua L. fruits. The phenolic compounds were extracted by four solvents of increasing polarity (dichloromethane, ethyl acetate, butanol and methanol) respectively and analyzed using different tests in vitro in order to evaluate their total phenolic and flavonoid contents, antioxidant and antibacterial activities. The phytochemical study of the c. siliqua fruits showed the presence of certain chemical compounds such as:  alkaloids, flavonoids, saponins, reducing compounds, tannins and volatile oils, comarins, triterpenes, resins and the total absence of imodols. The content of the total phenols by the Folin-Ciocalteu method and flavonoids by the AlCl3 method with the methanoic extract gave higher values which estimated: (42.65 ± 4.90 mg AGE / g Ext) and (8.83 ± 1.24 mg QE / g Ext) respectively.  The quantitative evaluation of the anti-radical activity has proved that methanolic extract is the most active (IC50 = 0.029 mg / ml); however, ascorbic acid (used as control) showed approximately equivalent activity (IC 50 = 0.017mg / ml), indicating the presence of compounds effective in the chemical composition of the plant that have a high capacity in reducing DPPH. The phenolic extracts from the fruits C. siliqua showed considerable activity against all bacterial tested.


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