This study included morphological and anatomical features, and the ecological and geographical distribution of Matricaria pubscenes (Asteraceae) plants, which were found to be annual herbaceous plants, branched at the base and flattening on the ground. That is a spontaneous plant distributed in North Africa, particularly in Algeria in the Saharan and Sub-Saharan regions. In the current investigation, the relationships of Matricaria pubscenes were examined using morphological, anatomical, and micro-morphological methods. The present study reported the detailed stem anatomy, epidermis micromorphology, environmental characteristics, and the geographical distribution. The anatomical structure of this species was studied using fresh materials.