The present study deals with the assessment of the total phenolics and flavonoids contents of different extracts of Chrysanthemum segetum namely hexane, chloroform (CHCl3), ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts. N-BuOH extract displayed the maximum phenolics content (185.50 ± 0.09 μg GAE/ mg of extract) followed by EtOAc extract (140.25±0.06). Conversely, the flavonoids content was found to be maximum in EtOAc extract (40.30 ±0.90 μg of QE/ mg extract) followed by n-BuOH extract (27.00 ± 0.30). The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging effect study reveals that the inhibition percentage (I %) increases with time and concentration where the values were found to be 76.70, 79.50, 73.65, and 36.50% for n-BuOH, EtOAc, CHCl3 and hexane extracts, respectively. IC50 of DPPH scavenging activity of the four extracts was found to be 26.50, 29.25, 34.70 and 59.20 µg/mL for n-BuOH, EtOAc, and CHCl3, respectively. The antimicrobial activity was assessed against a set of Gram-positive and Gram-negative bacteria as well as against the fungus Candida albicans to find out that the n-BuOH and EtOAc extracts exhibit potent activities against Gram-positive bacteria together with the fungus compared to the other extracts. In addition, Staphylococcus aureus was found to be more sensitive and Pseudomonas aeruginosa more resistant to the tested extracts.