This investigation was carried out to estimate the influence of Puncture vine, ashwagandha, and a mixture of both on male infertile rats. The biological experiment was designed to divide the rats into five groups. Group (1): Healthy rats (6 rats) were considered as control negative. Rats (24 rats) were treated with Cadmium Chloride to induce male infertility and they are reclassified into the groups, each group with six rats and received a basal diet. Group (2): Control positive. Group (3): received 5% Puncture vine. Group (4): fed on 5% Ashwagandha. Group (5): fed on a 5% mixture of both herbs. After 28 days, the levels of glucose, kidney functions, liver functions, lipids profile, AI, and different hormones as LH, testosterone, and FSH were determined in different rat groups and the results showed improvement in all parameters in different treated groups, especially in the case of the puncture vine diet 5% followed by a mixture of both puncture vine and ashwagandha, and finally, the group, which was fed on 5% Ashwagandha.It could be recommended that the rats fed on Puncture vine and ashwagandha showed improved functions of the organs and also, LH, testosterone, and FSH hormones in infertile rats.
