In vitro antagonistic activity was studied according to the method of confrontation by direct contact on culture medium, between Fusarium oxysporum f. sp. lycopersici and Trichoderma sp that have been considered as biological control agents against fungal diseases of plants. It could inhibit 95.09% from the mycelial growth of Fusarium oxysporum f. sp. lycopersici in comparison with test reference during seven days of the incubation at 25 ° C. Interesting results were also obtained in vivo: spraying tomato plants with a spore suspension of Fusarium oxysporum f.p. lycopersici and Trichoderma sp reduced the incidence of root and neck fusariosis compared to the untreated plants and inoculated ones by the pathogen. In addition, plants treated with Trichoderma sp had a greater vegetative development.