Production of crops using fertilizers plays an important role in agriculture. But the tremendous application of chemical fertilizers has a great influence on human health as well as the environment, hence nature demands the application of environment-friendly fertilizers. The main objective of the present study was to formulate the production of bio compost utilizing waste and non-edible vegetables along with spoiled fruits collected from Shahada market and household regions. The compost produced with waste vegetables and fruits was enriched with Azotobacter and Rhizobium inoculant along with sugar industry distillery effluent and cow dung. The resulting enriched bio compost was applied for the growth of wheat, mung and groundnut seeds by pot assay technique. Results obtained revealed that as compared to control seeds, a tremendous increase in the root and shoot length, plant height, chlorophyll content, and percentage germination was found in seeds treated with bio compost as compared to chemical fertilizer and control. The present study concluded that vegetable waste with microbial fertilizers and distillery effluent along the cow dung was found to be potential fertilizer for the growth of plants and environmental sustainability.