The purpose of this study is to quantify the bioactive substances contained in the stigmas of Algerian corn in the area Ain mlila, the sample was subjected to a maceration in the methanol, and the content of total phenols and flavonoids using the Folin-Ciocalteu method and the trichloride of aluminum respectively showed that the results vary between 91.51 ± 2.83 to 313.58 ± 0.01µg AGE/mg of extract for the polyphenols and between 11.26 ± 2.64 to 101.59 ± 0.02µg QE/mg of extracts for the flavonoids. These results show the richness of the stigma of corn studied in phenolic compound. The anti-radical activity has proved that our extract is more active (84.57%) than that of ascorbic acid (83.4%) used as control. It is found that reducing activity is strongly related to the content of polyphenols with R2 = 0.9997. Antibacterial activity was also tested on four bacterial strains (gram + and gram -) according to the disk diffusion method. No activity was reported on the reference strain Escherichia coli (ATCC 25921) and Staphylococcus aureus (ATCC 25923) and the two clinical strains tested.