The main purpose of this work was to analyze some biological and oxidative stress markers to predict and diagnose osteoporosis in postmenopausal women. For the experiment, we have chosen 20 healthy menopausal women as control and 20 menopausal women who had osteoporosis. Some biochemical, hematological, and oxidative stress parameters were measured. The sensitivity and specificity of oxidative stress biomarkers in serum, erythrocytes, and leucocytes were estimated using Receiver Operating Characteristics (ROC) curve design. The results of the study demonstrate a significant change (P<0.05) in biochemical and hematological markers during osteoporosis in post-menopausal women. In addition, results show a significant enhancement of MDA content and reduction in GSH, SOD, Catalase, and TAC levels in patients compared to control. Some oxidative stress markers identify as risk factors (P<0.05, OR>1) and represented an important significant specificity (P<0.05) for osteoporosis diagnosis such as serum MDA (AUC=99.6%), erythrocytic GSH (AUC=100%), and leukocytic SOD (AUC=62.5%), with a strong correlation (P<0.05) between GSH levels and serum calcium and ALP changes. In conclusion, several clinical factors contributed to the evolution of osteoporosis in postmenopausal women. Oxidative stress and hematologic markers represent very important diagnostic and predictive factors for osteoporosis in women during the post-menopause period.