Since natural resources for fossil fuels are getting harder to find, bioethanol has become one of the most promising and attractive liquid fuels. Bioethanol, which can be made from lignocellulosic materials, is attracting more and more attention because it is easy to get, cheap, environmentally friendly, and useful. Bioethanol is a suitable fuel substitute in our view. Bioethanol is a cleaner-burning fuel with the potential to match the performance of gasoline in internal combustion engines. Accordingly, a study was conducted on Alternanthera caracasana as a possible source of bioethanol due to its availability and abundance in nature. It is commonly called Khaki weed and is rarely used as a feedstock in animal husbandry and poultry. A. caracasana can be easily used for the extraction of lignocellulosic materials due to its abundance and non-usability. This lignocellulosic material can be subjected to fermentation for the production of bioethanol. The extracted bioethanol was characterized with FTIR and GC-MS for purity and percentage.