Since natural resources for fossil fuels are getting harder to find, bioethanol has become one of the most promising and attractive liquid fuels. Bioethanol, which can be made from lignocellulosic materials, is getting more and more attention because it is easy to get, cheap, environmentally friendly, and beneficial. Bioethanol is the perfect fuel substitute in our view. Bioethanol is a cleaner-burning fuel with the potential to match gasoline's performance in internal combustion engines. Accordingly, a study was performed on Alternanthera caracasana as a possible source of bioethanol because of its availability and abundance in nature. It is commonly called Khaki weed and in very few cases this is used as a feedstock in animal husbandry and poultry. A. caracasana can be easily used to extract lignocellulosic material because of its abundance and non-usability. This lignocellulosic material can be subjected to fermentation for the production of bioethanol. The extracted bioethanol was characterized with FTIR and GC-MS for purity and percentage.