The present study deals with the assessment of the total phenolics and flavonoids contents of different extracts of Chrysanthemum segetum namely hexane, chloroform (CHCl3), ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts. N-BuOH extract displayed the maximum phenolics content (185.50 ± 0.09 μg GAE/ mg of extract) followed by EtOAc extract (140.25±0.06). Conversely, the flavonoids content was found to be maximum in EtOAc extract (40.30 ±0.90 μg of QE/ mg extract) followed by n-BuOH extract (27.00 ± 0.30). The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging effect study reveals that the inhibition percentage (I %) increases with time and concentration where the values were found to be 76.70, 79.50, 73.65, and 36.50% for n-BuOH, EtOAc, CHCl3 and hexane extracts, respectively. IC50 of DPPH scavenging activity of the four extracts was found to be 26.50, 29.25, 34.70 and 59.20 µg/mL for n-BuOH, EtOAc, and CHCl3, respectively. The antimicrobial activity was assessed against a set of Gram-positive and Gram-negative bacteria as well as against the fungus Candida albicans to find out that the n-BuOH and EtOAc extracts exhibit potent activities against Gram-positive bacteria together with the fungus compared to the other extracts. In addition, Staphylococcus aureus was found to be more sensitive and Pseudomonas aeruginosa more resistant to the tested extracts.
This work is licensed under a Creative Commons Attribution 4.0 International License.
