Plant-based natural products serve as a source of medicine for curing many diseases and ailments since the existence of life. As per the recent investigation, approximately 25000 products from plants have been obtained which are being used in various diseases. Plants are recently being used in different biological activities due to their efficacy to treat serious diseases. So the study present has been planned to elucidate the chemical profiling and antimicrobial activity of Oleoresin from Chir-pine from Narendranagar forest division under Garhwal Himalaya. Oleoresin obtained from Chir-pine has been tested against Escherichia coli, Listea monocytegenes, and Staphylococcus aureus at three different concentrations Viz. 50 mg/ml, 100 mg/ml and 200 mg/ml. It was recorded that Oleoresin was active against all three tested bacterial cultures. We have tested all the Oleoresin extract in 50 mg/ml, 100 mg/ml, and 200 mg/mlof DMSO against the Escherichia coli, Staphylococcus aureus, Listea monocytegenes which showed significant activity. High activity was recorded in 200 mg/ml concentration with an inhibitory zone of 2.0± 0.34 mm against Staphylococcus aureus whereas minimum activity was recorded against Listea monocytegenes with an inhibitory zone of 1.2±0.30 mm.