This work aimed to establish the incidence of Preterm premature rupture of membrane (PPROM) and to identify the microbial organisms colonizing pregnant women with PPROM in the Western Province of Saudi Arabia. This retrospective study included all Saudi pregnant women diagnosed with PPROM who delivered at ≥27 weeks at King Abdulaziz University Hospital (KAUH), Jeddah, Saudi Arabia, from January 2016 to December 2020. To detect routine microbial pathogens, cultures were analyzed from maternal specimens. PPROM cases accounted for 1.07%. O of all deliveries during the study period. The predominant pathogen isolated was Group B streptococcus (GBS) (72.9%), followed by Candida (18.6%). Advanced maternal age, primiparity, and neonatal morbidity were associated with PPROM (P=0.001). There is a low incidence of PPROM in Saudi Arabia. GBS was the most common microbial pathogen isolated from Saudi women admitted to KAUH with PPROM. This profile, which differs from the findings from other regions, highlights a clear need to determine the PPROM-associated pathogens in each country or region to establish suitable management with antibiotics. More advanced techniques are needed to detect genital infections and prevent subsequent complications.