The three poxviruses' namely lumpy skin disease, Goatpox, and Sheeppox viruses are categorized under the genera of Capripoxvirus based on their genome similarities. The disease caused by these viruses is complex and difficult to diagnose; the most important reasons for this are the visible variability in the host range, the clinical similarities, and the inability to differentiate by serological assays. By considering their antigenic and immunogenic similarities of capripoxviruses, this study aimed at the comparison of the immunogenicity of GTPV A27R, GTPV L1R, and SPPV A33R recombinant proteins. Concerning this study, 35 kDa, 26 kDa, and 19 kDa recombinant proteins were purified as regards GTPV A27R, GTPV L1R, and SPPV A33R respectively. The best antibody titer was revealed by GTPV A27R with the test sensitivity of 90 %, 100 %, 90 %, and diagnostic accuracy of 100 %, 90 %, and 100 % for sera of Cattle, Sheep, and Goat correspondingly. Based on the available results GTPV A27R protein showed more immunogenicity while compared with the rest of the two proteins and further studies are required in the same area for the future.