The tremendous increase in population causes an increase in the world’s demand for food. Recent agricultural technology involves the applications of pesticides and chemical fertilizers for the rapid and healthy growth of plants. Excessive use of chemical fertilizers for the fast growth of plants causes pollution, environmental hazards as well as affects soil quality poorly. Chemical fertilizers mainly nitrogen, potassium, and phosphorous are though vital micronutrients for plants growth, they cause severe health hazards. Biofertilizers- microbial inoculants are environment friendly, the best alternatives for today’s agronomy. The use of bio-inoculants in agriculture improves the quality and fertility of the soil. In the present research, comparative applications of chemical fertilizers with biofertilizers were studied on the growth and yield of Arachis hypogaea (L.). The seeds of Arachis hypogaea (L.) treated with Azotobacter and Rhizobium biofertilizer separately and in combination with both organisms along with chemical fertilizer urea (T1-T7) were studied in the present investigation. Data obtained revealed that seeds treated with biofertilizers showed a significant increase in the morphological properties of plants as compared to seeds coated with chemical fertilizer and untreated seeds. Maximum root, shoot length, and the number of leaves were observed in treatment T4, while maximum plant height was observed in treatment T5. Analysis of variance also revealed the significant difference between the treatment of different fertilizers.