Lemons make up the bulk of citrus fruits, and they're frequently employed in the juice business. These enterprises generate a lot of garbage because these peels contain a wide range of bioactive components, they may be used to create a variety of value-added products that help to reduce pollution. This study aims to determine the antibacterial activity and phytochemical analyses of Citrus limon peels extracted with a solvent. The antibacterial activity of Citrus limon peels was tested against Gram-positive bacteria like Staphylococcus aureus and Enterococcus faecalis, as well as Gram-negative like Salmonella typhi, utilizing different solvents including acetone, ethanol, ethyl acetate, and petroleum ether. To determine whether specific bio-reactive chemicals are present or absent in solvent extraction, a phytochemical analysis was performed. Antimicrobial tests such as agar well diffusion assay, the minimum inhibitory concentration (MIC), and the minimum bacterial concentration were performed, and the results revealed that the ethanol extract of Citrus limon peel was more effective than peel extracts prepared with other solvents. Essentially digesting chemical and organic waste, enzyme bio cleaner is a type of natural cleaning product that includes certain numbers and types of bacteria, as well as enzymes and microbial nutrients. The primary objective of the research is to create a natural cleaning agent from citrus fruit utilizing lemon.